Objective
Function Penalty Weights for Hyb Oligos (Internal Oligos) |
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Steve Rozen, Helen J. Skaletsky
(1998) Primer3. Code available at
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Acknowledgments
The development of Primer3 and
the Primer3 web site was funded by
Howard
Hughes Medical Institute and by the
National
Institutes of Health,
National Human Genome Research Institute. under grants R01-HG00257
(to David C. Page) and P50-HG00098 (to Eric S. Lander).
We gratefully acknowledge the support
of Digital Equipment
Corporation, which provided the Alphas which were used for
much of the development of Primer3, and of Centerline Software,
Inc., whose TestCenter memory-error, -leak, and test-coverage
checker we use regularly to discover and correct otherwise latent
errors in Primer3.
Original design of this primer-picking
web site by Richard Resnick, who also is an author
of this site's documentation. Primer3's design is heavily based
on earlier implementations of similar programs: Primer 0.5 (Steve
Lincoln, Mark Daly, and Eric S. Lander) and Primer v2 (Richard
Resnick). Lincoln Stein championed the use of
the Boulder-IO format and the idea of making the Primer3 engine
a software component.